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Will a water-wettable column allow me to retain polar compounds? | Trust Your Science ep3

from Waters Corporation

In reversed-phase chromatography, water is a weaker solvent so you expect everything to be retained. What about polar compounds?

Retaining polar analytes on a reversed-phase (RP) column is challenging simply because a polar analyte does not want to interact with a non-polar reversed-phase surface. Many analytical scientists will look to using a weaker, 100% aqueous mobile phase as a way to promote retention for polar analytes on RP columns, and select columns solely on this attribute. Jonathan Turner and Kim Haynes from Waters investigate this idea, and seek to prove or disprove if increased polar analyte retention can truly be linked solely to columns that are 100% aqueous compatible.

Get help selecting the right column with our online column coach:

Or, turn to the Waters Column Advisor, a tool that recommends the most appropriate columns depending on the application requirements you need to consider or the column characteristics you are looking for.

See all columns for chromatography from Waters:

about 10 months ago

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