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Using Image Lab™ Software for Total Protein Normalization on Western Blots

from Bio-Rad Life Sciences

"For more info, visit Learn how to use Image Lab Software to normalize chemiluminescent band signals against the total protein load measured from a stain-free image of the same western blot with Bio-Rad's proprietary stain-free gel chemistry and the stain-free imaging capabilities of the ChemiDoc™ Touch, ChemiDoc MP, ChemiDoc XRS+, Gel Doc™ EZ, and Gel Doc XR+ Imaging Systems. Bio-Rad’s stain-free technology allows the immediate visualization of proteins across the whole gel without staining. Stain-free imaging is a sensitive, time-saving alternative to traditional Coomassie staining. Our stain-free technology also enables instant verification of protein transfer and the validation of western blotting data via total protein normalization, eliminating stripping and reprobing steps. In combination with stain-free imaging, Image Lab software yields quantitative results in contrast to relative normalization."

about 1 year ago

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