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scfv library screening

from Biotechnology11

The orientation of ligation of VL and VH is VL-linker- VH or VH-linker- VL. The antigen specificity of the two construction types is similar but they can lead to secretory expression in E.coli in different level. So you can choose any of the two construction orientations and then design primer by PCR method and sequence assembly type. Several PCR strategies are listed below:

about 2 years ago

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