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Removing Air Bubbles from the NGC™ Chromatography System

from Bio-Rad Life Sciences

"For more information, visit This video explains how to remove bubbles and/or reduce bubble formation in your NGC System. Tips to reduce bubbles include warming up your multiwave detector for 30 to 60 min before use and flowing buffer at 0.1–0.2 ml/min in the manual mode until a stable/flat baseline is achieved before starting a method. The system may also be flushed with 100% methanol or ethanol at purge speed, with columns out of line, to remove bubbles. The single-wavelength and multiwavelength detectors may be inspected for bubbles and cleaned if needed, and this video explains the steps required, from to removing the detector Flow Cell l from the NGC System and cleaning the flow cell with 2% Contrad 70. Bio-Rad's NGC System is designed to rapidly automate the purification of biomolecules. The flexible, modular, and economical design makes NGC Systems the instruments of choice for method development and scale-up. NGC Systems are available in six preplumbed, factory-tested configurations with two different flow rates. Each preconfigured system can be further customized and upgraded by adding valves, detectors, or pumps to meet specific application needs. Any system can be configured for operation at either high or low flow rate by simply changing the pump head on the system pump module. With the NGC System, a single hardware platform can be modified as needed to suit changing applications and scales. We hope this video addresses your questions; if you should have any more questions about any Bio-Rad product, please contact Bio-Rad Technical Support: email or phone 1-800-4-BIORAD (1-800-424-6723)."

about 3 years ago

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