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Rapid and Sensitive Identification of Host Cell Proteins with SWATH® LC-MS Acquisition

from Technology Networks Webinars

A robust, reliable, and high-performance LC-MS method using SWATH® Acquisition is presented here as an innovative and efficient technique for detecting and quantitatively monitoring multiple individual HCPs simultaneously.

This technique provides individual protein identities, rather than a number for the total sum amount of HCP, which is a limitation of current ELISA based methods. The high sensitivity and maximal parallelization of quantitative measurements made possible by SWATH acquisition makes it an invaluable solution for the quantitation of multiple low abundance CHO-HCPs in biotherapeutics.

Join us for this exciting webinar to learn how Stemcentrx has employed SWATH Acquisition and the TripleTOF® 6600 LC-MS system to dramatically increase the speed of acquisition of HCP data, with exceptional sensitivity.

Key Learning Objectives:

-Understand how LC-MS/MS can provide maximum multiplexing for HCP detection
-Learn how to set up and implement a sample, unbiased SWATH data acquisition strategy with -approximately 1 hour runtime
-Learn how HCP identification and quantitation at low ppm levels can be accomplished

Register for this webinar by copying and pasting the link into your browser:

about 4 years ago

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