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Myths about IP - Pre-clearing is necessary to get good results

from Thermo Fisher Scientific Cell Biology

Pre-clearing is necessary to get good results? In fact pre-clearing is not necessary with Invitrogen Dynabeads Magnetic Beads. Is it really necessary to pre-clear your sample prior to immunoprecipitation? Let's say you have made the assumption that some proteins will always bind nonspecifically to your solid phase. In order to remove these proteins, you need to pre-clear the sample, right? Watch the video and learn how you can avoid background without any pre-clearing.

about 2 weeks ago

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