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Isotopic Ratio Outlier Analysis (IROA): accurate metabolic profiling

from felice

Benefits of 95% and 5% 13C labeling: Eliminates false data - 95% and 5% 13C gives rise to a distinctive isotopic pattern easily differentiated from natural abundance non-biological artefacts and noise Mathematically calculable to enable computational analysis Removes variances - labeled and unlabeled species are subject to the same biases, thus increasing comparability of different measurements Enables identification – the number of carbons for each metabolite can be determined; carbon number and mass together greatly restricts the number of possible molecular formulae Facilitates detections of low intensity features - at natural abundance more that one isotopic peak is often not detectable or can be excluded as noise

about 1 month ago

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