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How to Optimize Cell Migration Scratch Assays with Effective Post-scratch Microplate Washing

from BioTek Instruments

This is a tutorial video about optimizing your microplate-based scratch migration assays by effectively washing samples after scratches have been formed. Post-scratch washing is an important step in the wound healing assay protocol, whether you are generating your scratches manually, or using the fully automated BioTek AutoScratch Wound Making Tool to efficiently create uniform scratches in either 24- or 96-well microplates.

When done correctly, manual washing of scratches will reduce problematic cellular debris from the microplate wells and produce cleaner results compared to omitting this step. However, manual washing is a time consuming process that can produce variable results.

For a more reliable and convenient solution, BioTek has developed a fully automated method to quickly and effectively wash either a 24- or 96-well microplate using the MultiFlo FX Multi-Mode Dispenser. With this approach, 3 wash cycles are performed in a fraction of the time required for manual washing, using aspiration and dispense steps optimized for the scratch assay.

Scratch Assay Starter Kit

MultiFlo FX Multi-Mode Dispenser


about 10 months ago

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