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How to detect your target proteins using chemiluminescent detection reagents

from Thermo Fisher Scientific Protein Biology

Chemiluminescent detection has been the method of choice for western blot analysis because of its high sensitivity, high signal-to-noise ratios, and versatility for use with film or digital imagers. Using this approach, a membrane is probed with an antibody that is conjugated to an enzyme, usually HRP, and a chemiluminescent substrate.

about 1 month ago

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