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High Quality Gene Expression from Precious Samples — A Simplified Workflow

from Bio-Rad Life Sciences

"For more info, visit http://www.bio-rad.com/yt/PrimePCR-PreAmp Obtaining enough starting material to complete multiple gene expression experiments can be challenging when working with precious and limited samples. Find out how Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto determines pluripotency and differentiation of limited human embryonic stem cells using Bio-Rad's SingleShot™ Cell Lysis Kit, SsoAdvanced™ PreAmp Supermix, and PrimePCR™ Validated Primer Panels. This simplified workflow allows for the gene expression analysis of hundreds of genes from as little as 100 human embryonic stem cells, and without the cumbersome step of isolating and purifying RNA. Bio-Rad's SingleShot Cell Lysis Kits provide a complete and fast solution for generation of cell culture lysates for use in qPCR reactions, without an RNA purification step. When combined with expertly designed and validated PrimePCR assays and PreAmp Supermix, researchers are now enabled to explore more gene targets from limited samples than ever before. For more information, visit http://www.bio-rad.com/en-us/category/amplification-pcr?WT.mc_id=sm-GXD-ww-pcrreagents_cat_vyt_20150608-Jp_CcJxLlBs"

about 1 year ago

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