When working with peptides, can you use the same sample preparation methods as small molecules? In #TrustYourScience, Kim and Jon test reversed-phase solid phase extraction (SPE), protein precipitation (PPT), and liquid-liquid extraction (LLE) protocols for two peptides, using greater than 80% recovery and less than 15% matrix effects as success criteria. Watch to see what they learn.
Analytical TechniquesChromatographyMass Spectrometry