Mapping Core Senescence Phenotype of Primary Human Colon Fibroblasts | Aging-US



February 29, 2024

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  • Aging-US published this research paper as the cover for Volume 16, Issue 4, entitled, "Mapping the core senescence phenotype of primary human colon fibroblasts" by researchers from the Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA; Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA; Division of Gastroenterology, University of Washington School of Medicine, Seattle, WA. DOI - Corresponding authors - William M. Grady -, and Ming Yu - Abstract Advanced age is the largest risk factor for many diseases and several types of cancer, including colorectal cancer (CRC). Senescent cells are known to accumulate with age in various tissues, where they can modulate the surrounding tissue microenvironment through their senescence associated secretory phenotype (SASP). Recently, we showed that there is an increased number of senescent cells in the colons of CRC patients and demonstrated that senescent fibroblasts and their SASP create microniches in the colon that are conducive to CRC onset and progression. However, the composition of the SASP is heterogenous and cell-specific, and the precise senescence profile of colon fibroblasts has not been well-defined. To generate a SASP atlas of human colon fibroblasts, we induced senescence in primary human colon fibroblasts using various in vitro methods and assessed the resulting transcriptome. Using RNASequencing and further validation by quantitative RT-PCR and Luminex assays, we define and validate a ‘core senescent profile’ that might play a significant role in shaping the colon microenvironment. We also performed KEGG analysis and GO analyses to identify key pathways and biological processes that are differentially regulated in colon fibroblast senescence. These studies provide insights into potential driver proteins involved in senescence-associated diseases, like CRC, which may lead to therapies to improve overall health in the elderly and to prevent CRC. Sign up for free Altmetric alerts about this article - Subscribe for free publication alerts from Aging - Keywords - aging, senescence, senescence associated secretory phenotype, SASP, colorectal cancer, cancer About Aging-US Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways. Please visit our website at​​ and connect with us: Facebook - X - Instagram - YouTube - LinkedIn - Pinterest - Spotify - Media Contact 18009220957 MEDIA@IMPACTJOURNALS.COM

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