Octet® RH16 and RH96 | High Throughput Monitoring of Binding of Proteins and Other Biomolecules

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November 22, 2023

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  • The Octet® RH16 and RH96 systems are the benchmark for high throughput monitoring of real-time binding of proteins and other biomolecules. Learn more at: https://www.sartorius.com/en/products/protein-analysis/octet-bli-detection The Octet® platform will improve the overall drug discovery process to increase the success rate of preclinical candidates and identify elite candidates earlier in screening. 16-channel Octet® RH16 is ideal for high-throughput applications that demand high sensitivity and low sample volume requirements. The 96-channel ultra-high-throughput Octet® RH96 monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed. From IgG titer to epitope binning, the Octet® platform offers label-free molecular interaction analysts with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules. 00:01 The Sartorius Octet® allows researchers to analyze protein-protein and protein-small molecule interactions and represents the industry-standard for label-free analyses. 00:13 The Octet® RH16 and RH96 systems are the benchmark for high throughput monitoring of real-time binding of proteins and other biomolecules. 00:26 With the ability to read up to 96 samples in parallel, titer determinations and kinetic screens are completed in just minutes, rather than hours. 00:35 High detection sensitivity makes these systems favorites for small molecule library and fragment screening, as well as analysis of large cross-competition binding matrices. 00:48 Target ligands can be individually, or batch immobilized onto biosensors, which can be regenerated and placed back into the biosensor tray for re-use in future experiments. 01:01 Key applications benefitting from the unique strengths of the Octet® RH systems include off-rate ranking for clone selection, titer determination and antibody competition. 01:14 The RH96 allows complete titer determinations for a 96-well plate of IgGs in just two minutes or provides 384 binding kinetics results in as little as 15 minutes. 01:28 You can also perform customized, multi-step quantification assays for applications such as host cell protein contaminations and residual protein A detection, allowing detection of sub-nanogram per milliliter concentrations. 01:45 These systems are also ideally suited to epitope binning experiments. For example, with the Octet® RH96, a 32 by 32 matrix can be completed in record time, with a dedicated epitope binning analysis function to enable easy visualization of data sets. The software also provides a flexible data matrix that can accommodate a variety of cross-blocking formats. 02:12 You can further increase productivity by using the systems’ high throughput capabilities to perform multiple assays in the same sample. For example, you can perform glycan screening and analyte titer determinations as early as the subcloning stage, helping to ensure only high-quality candidates continue downstream, and preventing late failures. 02:37 In summary, the Octet® RH16 and RH96 systems accelerate discovery, process development and manufacturing for biologics and vaccines, so more people will have access to better medicines, faster. Follow us on LinkedIn: https://www.linkedin.com/showcase/octet-affinity-binding-kinetics-and-concentration/ Do not forget to subscribe to have better access to our videos! #BioLayerInterferometry #BLI #Octet #GenuineOctet

    Analytical TechniquesBiopharmaBioprocessingDrug Discovery
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