Octet®️ Platform: BLI Applications



November 20, 2023

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  • Label-Free High-Quality Kinetics and Quantitation in Real-Time - The Octet®️ Bio-Layer Interferometry (BLI) platform is a comprehensive solution for screening and characterizing molecular interactions such as protein-protein or protein-drug interactions. Learn more: https://www.sartorius.com/en/products/protein-analysis/octet-bli-detection It enables an enviable variety of applications throughout the drug discovery and development process — from early selection to validation to manufacturing. Also, BLI-based analysis is widely used in biopharma and academic research. Octet®️ BLI label-free molecular interaction analysis provides critical information for specificity and affinity of biomolecular interactions for monitoring concentration and Critical Quality Attributes (CQA) in basic research, drug discovery, and bio-therapeutics manufacturing. Visit our BLI applications pages: https://www.sartorius.com/en/applications/life-science-research/label-free-detection 00:02 Bio-layer interferometry has applications throughout biomolecular analysis workflows and the drug discovery pipeline. 00:11 The Octet®️ range of BLI instruments offers benefits across the entire workflow, irrespective of the number of samples which need to be analyzed. 00:20 In the early phase of target ID and validation, the Octet®️ Bio-Layer Interferometry can be used to determine the nature of molecular interactions with your target molecule or receptor, helping to establish the mechanism of action for novel therapeutic candidates. 00:35 Affinity testing can then be used in candidate screening, using on- and off rate binding data to support lead selection. 00:43 The Octet®️ BLI is ideally suited to downstream optimization processes, such as high-affinity binding studies. 00:50 Its real-time, label-free approach to lead optimization provides crucial information on binding specificity and affinity, and allows you to monitor critical quality attributes during biologics development. Octet®️ BLI saves time and offers valuable insights to help characterize potential leads more efficiently. 01:10 Octet®️ biolayer interferometry systems also fit seamlessly into the preclinical development phase. The precise and sensitive nature of this technology makes it suitable for immunogenicity studies, where you can measure exact antibody responses after treatment or vaccination. 01:27 When a drug reaches the process development phase, the Octet®️ BLI can support numerous applications. For example, real-time target concentration measurements can be used to optimize cell line development, by aiding the selection of growth media and conditions. 01:44 Finally, the Octet®️ BLI instrument family can be used in a wide range of quality control applications, offering easy measurement of protein stability, potency and activity. 01:55 Whatever your application or stage in the drug development pipeline, the Octet®️ BLI platform provides a scalable solution for your biomolecular interaction studies. 02:06 In summary, Octet®️ biolayer interferometry (BLI) simplifies progress in life sciences and bioprocessing, enabling the development of new and better therapies, and more affordable medicines. Follow us on LinkedIn: https://www.linkedin.com/showcase/octet-affinity-binding-kinetics-and-concentration Do not forget to subscribe to have better access to our videos! #BioLayerInterferometry #BLI #Octet

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