Octet®️ Platform: Bio-Layer Interferometry (BLI) Technology Introduction

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November 20, 2023

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  • Label-Free High-Quality Kinetics and Quantitation in Real-Time - For over fifteen years, the industry-proven Octet®️ BLI platform has pioneered real-time, robust, label-free analysis for determination of affinity, kinetics and analyte quantitation. Learn more: https://www.sartorius.com/en/products/protein-analysis/octet-bli-detection Making the characterization of drug candidates significantly easier, faster and better! Octet®️ systems deliver greater value over traditional techniques with limited throughput and performance. Discover the fastest and most flexible system on the market with an enviable range of applications. 00:02 Science is continuously evolving. And so is the Octet®️ Bio-Layer Interferometry system. 00:10 Bio-Layer Interferometry technology, or BLI, has been providing researchers with a label-free approach to monitoring biomolecular interactions for over 15 years, overcoming the limitations in throughput, performance and cost associated with traditional technologies. 00:27 Accelerate your workflows and get the results you need to progress your research with Sartorius Octet®️. The path to progress just got simpler. 00:38 BLI is an optical technique that analyzes the interference pattern of white light reflected from two surfaces: a layer of immobilized protein on a biosensor tip, and an internal reference layer. Any change in the number of molecules bound to the biosensor tip causes a shift in the interference pattern, which is recorded in real time. 00:59 A proprietary protein coating at the biosensor tip acts as a binding partner, providing reproducible coupling of target molecules while minimizing non-specific binding. The measurement is plate-based, and aqueous buffer is used to generate a baseline, before the sensor dips into a sample containing the analyte. 01:18 The analyte then binds to the sensor surface, and the binding interaction is followed in real time to reveal the association kinetics. The sensor tip is then dipped into fresh buffer, and the dissociation can be monitored until the regeneration of the biosensor surface. 01:36 This real-time analysis provides precise and accurate data on binding specificities, analyte concentrations, and rates of association and dissociation. 01:48 A unique characteristic of BLI is that only analyte binding or dissociation can shift the interference pattern and generate a response profile. Unbound molecules, changes in the refractive index or pH, or other matrix effects do not affect results, making this technology well suited to measurements in crude samples. 02:09 In addition, BLI is easy to use, making assay development and optimization a much faster process, for a shorter time to results. This fluidic-free approach eliminates sample loading or cleaning steps between measurements. 02:23 Finally, BLI is easily scalable. With the sensors arrayed in a standard SLAS-format, you can perform measurements with multiple sensor tips in parallel to perfectly match individual labs’ throughput needs. Follow us on LinkedIn: https://www.linkedin.com/showcase/octet-affinity-binding-kinetics-and-concentration Do not forget to subscribe to have better access to our videos! #BioLayerInterferometry​ #BLI #OctetBLI

    Analytical TechniquesBiopharmaBioprocessingDrug Discovery
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