Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells

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ACEA Biosciences

The chromium release assay, a common assay for detecting cytotoxic T cell activity, has several limitations. Using antigen-specific CD8 T cells and the human breast cancer tumor line SKBR3, in the present article the xCELLigence real-time cell analysis instrument is examined for the capability of detecting cell killing. Results are directly compared with the traditional chromium-51 release assay.

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References:
Erskine, C. L., Henle, A. M., Knutson, K. L. Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System. J. Vis. Exp. (66), e3683, doi:10.3791/3683 (2012).