ERDC Sediment Spiking Methodologies for Nanomaterial Powders and Aqueous Suspensions for Bioassays



December 30, 2015

The present film was created by the US Army Engineers Research and Development Center in Vicksburg, MS for their Environmental Consequences of Nanomaterials Focus Area. For a step-by-step guide of the dispersion of nanoparticle used here, visit Homogenous dispersions of nanoparticles (NPs) for use in bioassay media for exposures/characterizations are crucial in ecotoxicology assays of nanomaterials in order to obtain reliable and repeatable results. Further, selection of the method for spiking sediment should consider the functional feeding behavior of benthic organisms, which impact the way it is exposed to NPs in the sediment; therefore, the present film provides step-by-step methods for spiking nanoparticle working stock suspensions into or onto the surface of sediment for bioassays based on test organism functional group.

Environmental MonitoringEnvironmental Science

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