Dual-fluorescent Calcein-AM/PI Assay for Cell Concentration and Viability

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Nexcelom Bioscience

A Cellometer assay principal illustration of a dual-fluorescent CalceinAM/PI assay for cell concentration and viability Calcein-AM penetrates the cell membrane of both living and dead cells. Once inside a living cell, Calcein-AM is hydrolyzed by cellular esterases into the cell membrane-impermeable Calcein. PI is only permeable to dead cells.