Human Cell-Free Expression System Generates Stable-Isotope-Labeled Protein Standards for Mass Spec

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Chromatography & Mass Spectrometry Solutions

Human Cell-Free Expression System Generates Stable-Isotope-Labeled Protein Standards for Mass Spec Speaker: John Rogers, Ph.D. - R&D Manager, Mass Spectrometry Reagents John Rogers presents his scientific poster about human cell-free expression system for the production of stable-isotope-labeled (heavy) proteins using HeLa cell lysates. Six different stable-isotope-labeled proteins were produced. Isotope incorporation efficiency of greater than 95% was observed for IVT reactions. Thermo Scientific LTQ Orbitrap XL ETD Spectrometer provides a comprehensive solution for protein ID, characterization and quantitation. A powerful, comprehensive instrument for proteomics analyses.