Expressioneering™ Technology: surprisingly simple recombinant protein expression



August 1, 2011

Clone your favorite gene in an effortless afternoon, and start expression analysis the next day with the Expresso® Cloning and Protein Expression Systems, available with Rhamnose or T7 promoters and SUMO fusion tag to improve protein solubility. Expressioneering™ Technology uses in vivo homologous recombination to seamlessly clone PCR amplified DNA into specially designed expression vectors without the need for enzymes or purification steps. The desired insert is simply amplified with primers that include 18 bases that overlap with the ends of the Expresso vector. The unpurified PCR amplicon is then mixed with the pre-processed expression plasmid and the high-efficiency competent cells provided, and directly plated on appropriate media. Features of the Expressioneering Technology: • No vector preparation • No restriction enzymes or ligase required • No DNA purification steps • Tightly-controlled expression of N- or C-Terminal 6xHis-tagged proteins • Smallest vectors available for easier downstream manipulation (2.2 kb to 2.5 kb)

Flow ChemistryGenomicsInformaticsMolecular Biology

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