Optical Sensors for Quantification of Intracellular Redox Potentials

3,296 views

|

October 22, 2013

Redox signaling is important in determining cell fate decisions and plays a pivotal role in the pathology of diseases such as cancer and neurodegeneration. There are significant challenges in quantitatively measuring intracellular redox potentials, and in this presentation, I will introduce a new approach. This approach is based on the use of nanosensors which comprise molecules that sense the local redox potential, assembled on a gold nanoshell. Since the Raman spectrum of the sensor molecule changes depending on its oxidation state and since the nanoshell allows a huge enhancement of the Raman spectrum, intracellular potential can be calculated by a simple optical measurement. The nanosensors can be controllably delivered cells, without any toxic effects, allowing redox potential to be monitored in a reversible, non-invasive manner over a previously unattainable potential range encompassing both superphysiological and physiological oxidative stress. In this presentation, the concept, the reporter molecules and the technique will be introduced. Furthermore, I will discuss how we have used this approach to study the regulation of redox potential in apoptosis and have correlated redox potential changes with changes in caspase activity.

MicrofluidicsSpectroscopy

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.