Electrochemical and Optical Multiple Parameters Genosensors: Tools for Disease Predisposition Analysis
3,374 views
|
July 23, 2012
Valerio Beni, Assistant Professor, Biosensors & Bioelectronics Centre, Linkoping University
Abstract
Multiple parameters genosensors are of growing interest in the field of clinical analysis where they find application in the early diagnosis of genetic diseases or as support in the diagnosis of genetically associated diseases. Coeliac disease is a small intestinal inflammation triggered by the intake of gluten. This disease has been shown to affect only genetically predisposed individuals. Strong relation between Coeliac disease and two Human Leukocyte Antigens (HLA) genes, DQ2 and DQ8, has been reported, with almost 100% of the affected patients carrying at list one of them. In this seminar an electrochemical and a fluorescence genosensors, both for the low to medium resolution typing of Coeliac disease associated HLA genes, are presented. The fluorescence genosensor was based on the integration of gold nano-particles and molecular beacons whilst the electrochemical genosensor was based on an enzymatic sandwich assay format performed at photolitographically fabricated electrode array. The optimisation of different aspects involved in the sensors preparation and real sample detection,as surface chemistry, assay conditions, probe's design and single stranded DNA generation, is presented. This optimisation was achieved by the combination of enzyme linked oligonucleotides assay, surface plasmon resonance, electrochemistry and fluorescence measurements.
