The Influence of Structures on Cellular Processes During Differentiation of neuronal Stem Cells - Adhesion
3,525 views
|
July 17, 2012
Clemens Holzer, Head of Department, University of Leoben
Abstract
Stem cell differentiation can be driven by the material /cell interface which allows for a , chemistry independent manipulation of stem cells. Identification of polymers and surfaces , that modulate stem cell fate have remained elusive. It was our aim, to identify specific , polymers and nanostructures that modulate proliferation and/or viability of neuronal stem. The neuronal stem cell line Ntera-2 was cultured on COP, PMMA, PS, PP, PLA, PC for four days. Adhesion and viability of Nteras was significantly increased when cells were cultured on corona or plasma treated COP and PMMA as compared to PS culture dishes from Nunc or Costar (242,72%, p = 0,001; 227,78 %, p = 0,0001 respectively). Hydrophilicity of treated surfaces was evaluated by measuring surface contact angles. Increased viability of cells correlated with a decreased contact angle (r = -0,87). Our results indicate that commercially available synthetic materials such as PS dishes are suboptimal for stem cell culturing. Corona treated COP and PMMA culture plates were further processed and implemented for nanostructure studies. Subsequent to culturing on nanoridges Nteras were found to align with the striations to a bipolar cell type. We will analyse the effect of structure induced modulations of stem cells in more detail.
