Translating Genetic Variations into Fluorescent Signatures using Lights-On/Lights-Off Probes



October 18, 2011

Life abounds with genetic variations writ in sequences that are often only a few hundred nucleotides long.  Rapid detection and use of these variations has become the mainstay of molecular science and medicine for identification of organisms, pathogens, and mutations.  My presentation will describe a new highly informative closed-tube PCR strategy for analysis of both known and unknown sequence variations.   It combines efficient quantitative amplification of single-stranded targets via LATE-PCR with pairs of Lights-On/Lights-Off probes.  Together these technologies generate sequence-specific fluorescent signatures over a wide range of detection temperatures and sequence length. Contiguous pairs of Lights-On/Lights-Off probes of the same colour can be used to scan DNA targets several hundred nucleotides long, while sets of probes in different colours can be used to simultaneously analyze different regions of one or more single-strands amplified in the same closed-tube reaction.  We anticipate widespread use of these exciting new technologies for diagnostics, species identification, and basic research.

Drug DiscoveryGenomicsInformaticsPersonalized Medicine

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