Direct Cellular Patterning and Analysis Using a Novel Microprinting Technology

3,875 views

|

June 1, 2011

  • Share
  • Patterning of living cells on solid surfaces has applications for cell-based screening assays, tissue engineering, cell signaling studies, and for the development of hybrid nanodevices and biosensors. To this end, we have developed a direct, live-cell printing technology for high throughput screening and analysis of bacterial and mammalian cells. This novel approach augments traditional microarray technology which has been extensively used to study proteins and nucleic acids. Micro-scale “quill-pen” cantilevers have been developed to transfer living cells onto a wide variety of surfaces in a variety of matrices. In contrast with existing cell deposition methods, such as ink jet or laser ablation, the quill-pen approach imparts minimal thermal and shear stress to cells, preserving cell viability and biological functionality. Cell viability and proliferation has been evaluated, demonstrating the feasibility of this printing method and its compatibility with a wide range of cell types.

    Molecular Biology

    Keep up to date with all your favourite videos and channels.

    Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.