Western Blot Visual Protocol: Phase 1: Sample Preparation

Novus Biologicals Visual Protocols: In phase 1 of the western blot procedure, you will learn how to prepare your samples before loading them into a gel. Here we isolate protein from cultured cells, quantify total protein concentrations with a BCA assay, add loading buffer to the sample, and heat the sample. Additional help can be found in the support section of www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.