Combined RNA & DNA Library Preparation for NGS-In a Single Tube

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October 17, 2023

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  • In this informative Teach me in 10, Mariana Kiehl, a Senior Applications Scientist at Roche Sequencing & Life Science, introduces a novel single-tube workflow designed to seamlessly combine RNA and DNA sequencing from the same starting sample. She elucidates the intricacies of this method, highlighting its remarkable potential for providing a more profound insight into the intricate biological processes occurring within cells. Utilizing FFPE tumor tissue sequencing as a compelling case study, Dr. Kiehl elucidates how this innovative approach conquers several common challenges that often impede the success of concurrent sequencing. These challenges encompass limited tissue availability for analysis, the inherent cellular diversity found in many tumors (even within adjacent sections), and the time-consuming need for separate DNA and RNA workflows, which can significantly extend the sample processing and analysis duration. Experience the comprehensive breakdown of this cutting-edge technique by watching this Teach Me in 10.

    InformaticsNext-Generation SequencingProteomics and Metabolomics

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