Antibody protein is initially digested into overlapping peptides using various trypsin Digestion with LC-MS/MS, then fragmentation optimization Overlapping peptides are characterized by de novo peptide sequencing Automated antibody sequence assembly using characterized peptides Data analysis is performed using state-of-the-art computational algorithms that efficiently derive the amino acid sequence of the digested peptide https://www.creative-biolabs.com/next-generation-antibody-sequencing.html
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