Reporter Stem Cells Generation

Reporter stem cell lines are valuable models which enable noninvasive, live monitoring of marker onset and expression in a cell-specific manner. Some methods have been used to derive such cell lines based on lineage promoter-driven reporter expression. A more regulated expression achieved with a reporter knock-in into the endogenous promoter loci was less utilized due to the associated technical difficulty. While new advances in genome-editing technologies have lowered these barriers for creating knock-in reporter lines.