An Overview of NGS Adapters for Ligation-based Library Preparation

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Teach Me in 10

In this Teach me in 10, Senior Field Application Scientist Nick Hapshe explains: (1) What NGS adapters are, and how they are used in library construction; (2) what sequencing indexes are, and why they are important; (3) How different types of adapters and indexing strategies can improve the quality of NGS data while reducing sequencing costs; and (4) What unique molecular identifiers (UMIs) are, and how they can increase accuracy in NGS data.