Generation of Reporter Stem Cells

Gene reporter is a useful in vitro tool that facilitates live monitoring and tracking a cell type of interest. Lineage reporters are created using minimal lineage-specific promoter-driven reporter systems or via knock-in of the reporter into the endogenous promoter region. Choice of reporter is critical based on intended application. Although there is a wide choice of reporters that can be used, each system has its own advantages and disadvantages. Green fluorescent protein (GFP) and variants, such as yellow fluorescent protein (YFP), cyan fluorescent protein (CFP) and DsRed, have been widely used to visualize cells. https://www.creative-biogene.com/support/genome-editing-generation-of-reporter-stem-cells.html