Single-cell Transcriptomics of Peripheral Blood in the Aging Mouse



January 16, 2023

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  • Aging-US published this research paper as the cover for Volume 15, Issue 1, entitled, "Single-cell transcriptomics of peripheral blood in the aging mouse" by researchers from the Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI; Center for Computational Molecular Biology, Brown University, Providence, RI; Center on the Biology of Aging, Brown University, Providence, RI. DOI - Corresponding author - Nicola Neretti - Abstract Compositional and transcriptional changes in the hematopoietic system have been used as biomarkers of immunosenescence and aging. Here, we use single-cell RNA-sequencing to study the aging peripheral blood in mice and characterize the changes in cell-type composition and transcriptional profiles associated with age. We identified 17 clusters from a total of 14,588 single cells. We detected a general upregulation of antigen processing and presentation and chemokine signaling pathways and a downregulation of genes involved in ribosome pathways with age. In old peripheral blood, we also observed an increased percentage of cells expressing senescence markers (Cdkn1a, and Cdkn2a). In addition, we detected a cluster of activated T cells exclusively found in old blood, with lower expression of Cd28 and higher expression of Bcl2 and Cdkn2a, suggesting that the cells are senescent and resistant to apoptosis. Sign up for free Altmetric alerts about this article - Keywords - aging, single-cell transcriptomics, senescence, peripheral blood About Aging-US Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways. Please visit our website at​​ and connect with us: SoundCloud - Facebook - Twitter - Instagram - YouTube -​ LinkedIn - Pinterest - Media Contact 18009220957 MEDIA@IMPACTJOURNALS.COM

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