Bioinformatics Analysis of 16s rRNA Amplicon Sequencing Data

CD genomics can provide microorganism quantification amplicon sequencing based on 16S external standard sequences to obtain absolute abundance data for species in the sample. The 16S amplicon library is constructed and sequenced by adding a synthetic sequence of known copy number to the sample DNA, and then a standard curve is drawn according to the number of amplicon reads and the absolute copy number of the external standard sequence. And finally the absolute copy number of the 16S rRNA gene of the corresponding species of the OTU representative sequence in the sample is calculated. More information at https://www.cd-genomics.com/absolute-quantitative-16s-18s-its-amplicon-sequencing.html