The 5 steps of a solid phase extraction (SPE)

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SiliCycle Inc.

Solid-phase extraction (SPE) is a technique designed for rapid sample preparation, purification and/or concentration prior to chromatographic analysis. SPE uses a sorbent that has a strong affinity for the desired compound and solvent changes to collect the individual components as single fractions. After applying the sample to a pre conditioned column, the column is eluted with a solvent that has a weak affinity for the desired product to removed impurities. It is then eluted with a solvent that has a strong affinity for the desired product thereby displacing it from the sorbent. Common sample matrices include: • human and animal biological fluids • waste waters • petrochemical residues • toxicological residues To check out SiliCycle's SiliaPrep SPE product line and the various sorbents available, visit: https://www.silicycle.com/products/silicycle-sample-preparation-products/spe-cartridges To see different applications and their protocols, you can find case studies in our Sample Prep Brochure: https://www.silicycle.com/media/brochure/silicycle-BROSAM-brochure-sample-preparation-web.pdf