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HPLC Analysis in a nutshell - How a sample is analyzed

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May 11, 2022

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  • Get a quick overview on how a sample is analyzed using High Performance Liquid Chromatography (HPLC). As the sample mixture in the mobile phase is pumped into the column, the analytes or compounds in it interact with the stationary phase (ie sorbent). The overall interactions make it that the compounds get retained with varying strength, thereby affecting when they exit the column. The detector at the exit records this elution and the information generates the chromatogram. Have more questions regarding HPLC? SiliCycle has compiled a list of the most frequently asked questions here: https://www.silicycle.com/faq/hplc If new to HPLC, it is also important to note that keeping the column in top condition with proper care will help to maintain column efficiency over an extended period of time, so you can continue your testing for as long as possible. This Application Note shares different procedures used to help extend the lifetime of HPLC columns. https://www.silicycle.com/resource-center/application-notes/hplc-columns-cleaning-regeneration-procedures

    Analytical TechniquesChemistryChromatography

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