Quick Tips: How to Choose a Primary Antibody for Multiplex Western Blotting



March 1, 2022

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  • In this video, we discuss the selection of primary antibodies. - Choosing primary antibodies from different host species is often the easiest way to multiplex. - When choosing antibodies from different host species, distantly related species are less likely to have issues with species cross-reactivities. Avoid choosing antibodies from closely related species like mouse and rat - Using primary antibodies from the species but different antibody classes like IgG and IgM in combination with appropriate secondary antibodies is one way to overcome species issues - Primary antibodies from the same species but of different isotypes such as IgG-1 and IgG-2 provide another possible solution Learn more at https://www.bio-rad.com/QuickTips We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Online: Website: http://www.bio-rad.com/ LinkedIn: https://www.linkedin.com/company/1613... Facebook: https://www.facebook.com/biorad/ Twitter: https://twitter.com/BioRadLifeSci Instagram: @BioRadLabs Snapchat: @BioRadLabs ©2021 Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of their respective owner. Music used by permission granted by royalty-free license from Pond5.com

    ImmunologyMolecular BiologyNeuroscienceProteomics and Metabolomics

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