Quick Tips: How to Choose Secondary Antibodies for Multiplex Western Blotting

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Bio-Rad Laboratories

In this video, we discuss the best practices for selecting secondary antibodies. - Use the correct secondary antibodies to detect your primary antibodies. This will be a species-specific, class-specific, or isotype-specific approach depending on your multiplexing approach. - Do not use secondary antibodies raised in the same species as any of your primary antibodies when using a species-specific approach. - Ensure specificity of your staining by using cross-adsorbed secondary antibodies to prevent cross-reaction with primary antibodies. The cross-adsorbed antibody will correctly bind the target primary antibody but will not cross-react to the primary antibody from the related species. - Ensure that the different detection methods can be detected by your imager. To further support your secondary antibody choice, download our secondary antibodies guide. Learn more about secondary antibodies at - https://info.bio-rad.com/Secondary-Antibodies-New-Practical-Guide.html Learn more about fluorescent western blotting at - https://www.bio-rad.com/en-us/category/fluorescent-western-blotting?ID=PMJ5J7TU86LJ We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Online: Website: http://www.bio-rad.com/ LinkedIn: https://www.linkedin.com/company/1613... Facebook: https://www.facebook.com/biorad/ Twitter: https://twitter.com/BioRadLifeSci Instagram: @BioRadLabs Snapchat: @BioRadLabs ©2021 Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of their respective owner. Music used by permission granted by royalty-free license from Pond5.com