Detecting Endogenous Nucleotides From Low Numbers of Mammalian Cells

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January 27, 2021

Biomedical laboratories are under increasing pressure to produce meaningful data from minute amounts of sample. In this webinar, we’ll explore how a micro-scale separation technique, capillary electrophoresis-mass spectrometry (CE-MS), can be used for the highly efficient and sensitive profiling of nucleotides from a low number of mammalian cells. Endogenous nucleotides can be detected in extracts as low as 500 HepG2 cells. In a research study discussed in this webinar, an injection volume of 6.5 nL resulted in sub-nanomolar detection limits for nucleotides, clearly illustrating the utility of this technique for metabolic profiling in low amounts of biological material.

Cell ScienceMass SpectrometryProteomics and Metabolomics

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