Hyaluronan (HA) Synthesis Inhibition Normalizes Hepatic Fibrogenic Features by Reducing Hepatic Stellate Cell Activation in Alcohol-Associated Liver Disease Models
Presenters: Michele Pritchard, Ph.D., Associate Professor, University of Kansas Medical Center and Maciej Czerwinski, Ph.D., Director of Consulting, SEKISUI XenoTech
In 2018, Dr. Pritchard published an abstract suggesting ethanol exposure sensitizes hepatic stellate cells (HSC) to activation stimuli resulting in enhanced pathogenic hyaluronan (HA) production. Targeting HSC hyaluronan production may therefore attenuate liver disease progression in alcohol-associated liver disease (ALD) patients.
Alcohol-associated liver disease (ALD) remains a pharmacologically incurable disease. Hyaluronan (HA), an extracellular matrix glycosaminoglycan, is increased in livers and blood of advanced liver disease patients who are actively drinking compared to those who do not drink. In the liver, hepatic stellate cells (HSCs) are responsible for most HA production, and HA synthesis is a prerequisite for fibroblast to myofibroblast transition. Further, increased HA content and fragmentation drive inflammation and fibrosis in many tissues. In this keynote, Dr. Pritchard will discuss how she and her colleagues tested the hypothesis that ethanol facilitates HSC activation through hyaluronan production in early ALD.
As a complementary presentation to Dr. Pritchard’s keynote, our in-house Director of Scientific Consulting, Dr. Maciej Czerwinski, will provide insight into pertinent test systems. SEKISUI XenoTech’s Research Biobank provides researchers with access to tissue microarrays (TMA) and liver tissue samples in various formats suitable for liver disease research. As a contributing author to Dr. Pritchard’s published abstract, and upcoming peer-reviewed publication, and resident expert for disease-state test systems, Dr. Czerwinski will provide details of the materials used and present an overview to the offerings and applications of Research Biobank tissue.
Key concepts discussed in the webinar:
- Hyaluronan (HA) role in alcohol-associated liver disease (ALD)
- Ethanol facilitation of hepatic stellate cell (HSC) activation through HA production early in ALD
- Inhibiting HA synthesis in ALD mouse models
- Tissue microarrays (TMAs) to evaluate HA and collagen content in steatotic livers
- Research Biobank tissue applicability
Download slides: https://www.xenotech.com/wp-content/uploads/2020/09/SEKISUI-XenoTech_Hyaluronan-Synthesis-Inhibition-Normalizes-Hepatic-Fibrogenic-Features.pdf
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About the Keynote Presenter:
Dr. Michele Pritchard is an Associate Professor with tenure at the University of Kansas Medical Center in the Department of Pharmacology, Toxicology, and Therapeutics, and is also Associate Director of the Interdisciplinary Graduate Program in Biomedical Sciences. She received her Ph.D. in Immunology from the University at Buffalo in 2003. She has authored or co-authored more than 40 peer-reviewed publications in scientific journals including International Journal of Toxicology, Journal of Histochemistry & Cytochemistry, American Journal of Physiology. Gastrointestinal and Liver Physiology, and Hepatology. Her current research involves exploring hyaluronan biology in regenerative wound repair and fibrosis after tissue injury and during physiologic aging. The aim of Dr. Pritchard’s research is to identify ways to prevent organ fibrosis and to improve wound repair by targeting hyaluronan.