Phosphorylation analysis by mass spectrometry

Protein phosphorylation, a reversible process, is characterized by adding phosphate donated from ATP and removing phosphate from a phosphorylated protein substrate. And the protein phosphorylation is catalyzed by protein kinase and phosphatase (PP). In eukaryotic cells, about 86.4% of the protein phosphorylation events occur on serine (Ser or S), while 11.8% protein phosphorylation events occur on threonine(Thr or T) whereas only 1.8% on tyrosine (Tyr or Y) residues. https://www.creative-proteomics.com/