How to detect your target proteins using chemiluminescent detection reagents

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September 1, 2019

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  • Chemiluminescent detection has been the method of choice for western blot analysis because of its high sensitivity, high signal-to-noise ratios, and versatility for use with film or digital imagers.

    Using this approach, a membrane is probed with an antibody that is conjugated to an enzyme, usually HRP, and a chemiluminescent substrate.
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