Preparative high pressure chromatography has no essential difference with traditionally classical column chromatography. It has higher efficiency and achieves automatic operation. About the instrument, a high pressure pump is adopted to deliver mobile phase. At the same time, a detector is connected to the outlet of the column which can detect the fraction on-line constantly.
The preparative high pressure chromatography has several features as following:
1.shortter column length;
2. larger column ID;
3. higher flow rate.
Sometimes it is also called High performance preparative liquid chromatography