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FireflySci - How to Calibrate a Spectronic 20D Spectrophotometer



February 28, 2019

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  • Hi Everyone! Learn how to properly calibrate your Spectronic 20D spectrophotometer with this easy method. Here is a video that we made after our trip to Hofstra University. We were called in to demo our calibration standards and we couldn't figure out how to get the machine to work. So they let us borrow a spectrophotometer and after some experimenting, we figured out how to perform the calibration. If you have any questions, please e-mail us at


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