Western Blotting Webinar: the principles and a comparison of indirect vs. direct immunodetection

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October 28, 2018

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  • Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture. Although Western blotting is simple in principle, for a successful outcome, scientists must be aware of the technical caveats which must be overcome. Therefore, to provide further insights in to Western blotting, this exciting webinar discusses:

    • Sample preparation
    • SDS-PAGE
    • Western Blot transfer
    • Blocking
    • Indirect Detection -- primary antibody incubation and indirect detection
    • Indirect Vs. Direct antibody detection -- advantages and disadvantages
    • Direct detection for multiplex fluorescent Western blotting
    • Problems with sourcing labeled antibodies for direct detection
    • The solution simple antibody labeling kits
    • Questioning the secondary antibody amplification hypothesis

    This video was produced by Innova Biosciences which is now fully integrated with Expedeon Ltd.

    - To find out more about our range of products designed for Western blotting applications, please visit: https://bit.ly/2xGxpH5
    - To learn more about Western blotting applications, feel free to download our guide: https://bit.ly/2R0wlqa

    Cell ScienceImmunology

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