CRISPR/Cas9 system consists of a “guide” RNA (gRNA) and a bacterial CRISPR-associated endonuclease (Cas9). The gRNA is a short synthetic RNA composed of a Cas9-binding “scaffold” sequence and ∼20 nucleotide “targeting” sequence that defines the target genomic site to be modified. https://www.creative-biogene.com/Services/Stable-cell-line-generation/Custom-Genome-Editing-Cell-Lines.html
Cell Science