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The V3 Western Workflow™ with ChemiDoc™ MP Imaging System

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February 23, 2017

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  • "For more info, visit http://www.bio-rad.com/SeeV3 This video illustrates the five-step process of Bio-Rad's V3 Western Workflow System, which enables you to not only streamline your western workflow but also visualize, verify, and validate your results at multiple points throughout the process. By combining traditional blotting techniques with innovative tools such as TGX Stain-Free™ Precast Gels, the Trans-Blot® Turbo™ Rapid Blotting System, and the ChemiDoc MP Imager, this system allows you to quickly check electrophoresis results and blot transfer quality prior to western blotting, simplifying total protein quantitation analysis post-immunodetection. Obtain consistent results faster and more easily than ever before. The Five Steps of the V3 Western Workflow system: • Separate proteins — Mini-PROTEAN® and Criterion™ TGX Stain-Free precast gels offer superior protein separation in as little as 15 minutes in standard Tris/glycine running buffer • Visualize proteins — protein separation can be visualized and confirmed on a ChemiDoc Touch Imager after a 1-minute activation without staining. Stain-free technology is a sensitive, time-saving alternative to traditional Coomassie staining, offering a streamlined workflow • Transfer proteins — the Trans-Blot Turbo system enables rapid protein transfer in as little as 3 minutes while maintaining high transfer efficiency over a wide range of molecular weights • Verify protein transfer — the ChemiDoc MP imager paired with stain-free technology enables instant verification of protein transfer to the membrane • Validate and quantitate — the ChemiDoc MP imager and Image Lab™ software can be used to validate western blotting data by total protein normalization to housekeeping proteins, eliminating the need for stripping and reprobing and preventing problems with oversaturation of control bands. Image Lab software yields quantitative results instead of relative normalization http://www.bio-rad.com/en-us/product/v3-western-workflow?WT.mc_id=sm-pqd-ww-v3-vyt-20161222-RWsDtTqDW_o"

    Analytical Techniques

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