V3™ Stain-Free Workflow for ImprovedTotal Protein Loading Control in Western Blotting

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February 23, 2017

"For more info, visit http://www.bio-rad.com/yt/8/v3. ""Seeing is believing, measuring is knowing"". Bio-Rad's V3 Western Workflow provides researchers with more confidence in the blotting process and results. This video demonstrates the fast, convenient, and transparent V3 western workflow process, how Image Lab software performs total protein normalization, and how stain-free technology is used to yield more reliable western blot results. The Five Steps of the V3 Western Workflow™ system: • Separate proteins — Mini-PROTEAN® and Criterion™ TGX Stain-Free precast gels offer superior protein separation in as little as 15 minutes in standard Tris/glycine running buffer • Visualize proteins — protein separation can be visualized and confirmed on a ChemiDoc™ MP imager after a 1-minute activation without staining. Stain-free technology is a sensitive, time-saving alternative to traditional Coomassie staining, offering a streamlined workflow • Transfer proteins — the Trans-Blot® Turbo™ system enables rapid protein transfer in as little as 3 minutes while maintaining high transfer efficiency over a wide range of molecular weights • Verify protein transfer — the ChemiDoc MP imager paired with stain-free technology enables instant verification of protein transfer to the membrane • Validate and quantitate — the ChemiDoc MP imager and Image Lab™ software can be used to validate western blotting data by total protein normalization to housekeeping proteins, eliminating the need for stripping and reprobing. Image Lab software yields quantitative results instead of relative normalization http://www.bio-rad.com/en-us/product/v3-western-workflow?WT.mc_id=yt-lsd-ww-v3-20140207-X186MkqnQvg"

Analytical Techniques

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