Revealing Breast Cancer Pathways with the V3 Western Workflow™



February 23, 2017

"For more information, visit Research led by Prof. Ryan Jensen at Yale Medical School focuses on elucidating the links between BRCA2 gene mutations and breast cancer. Learn how researchers in his lab use Bio-Rad’s V3 Western Workflow to study interactions between BRCA2 proteins and the DNA repair protein RAD51. Understanding how these proteins interact in carcinogenesis and tumor growth will provide a new understanding of breast cancer mechanisms that could lead to new therapies. The Five Steps of the V3 Western Workflow™ system: • Separate proteins — Mini-PROTEAN® and Criterion™ TGX Stain-Free precast gels offer superior protein separation in as little as 15 minutes in standard Tris/glycine running buffer • Visualize proteins — protein separation can be visualized and confirmed on a ChemiDoc™ MP imager after a 1-minute activation without staining. Stain-free technology is a sensitive, time-saving alternative to traditional Coomassie staining, offering a streamlined workflow • Transfer proteins — the Trans-Blot® Turbo™ system enables rapid protein transfer in as little as 3 minutes while maintaining high transfer efficiency over a wide range of molecular weights • Verify protein transfer — the ChemiDoc MP imager paired with stain-free technology enables instant verification of protein transfer to the membrane • Validate and quantitate — the ChemiDoc MP imager and Image Lab™ software can be used to validate western blotting data by total protein normalization to housekeeping proteins, eliminating the need for stripping and reprobing. Image Lab software yields quantitative results instead of relative normalization WT.mc_id=yt-lsd-ww-v3-20131306-LDnW_hprmPc"

Analytical Techniques

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