Author Interview: Phasing Genomes with Droplet Digital™ PCR

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February 22, 2017

"For more information, visit http://www.bio-rad.com/yt/Drop-Phase A new Droplet Digital PCR-based technology, Drop-Phase, can be used to rapidly determine whether genomic sequences are linked. We review the PLOS ONE paper that introduced this technique and speak with its lead author, John Regan. Droplet Digital PCR technology is the most precise and sensitive digital PCR solution for a wide variety of applications, featuring flexible assay chemistry optimized for both hydrolysis probe and dye-based assays. Nanoliter droplet partitioning reduces bias from amplification efficiency differences and PCR inhibitors and enables exquisitely accurate target quantification in a convenient assay design, without the use of standard curves. The QX200™ Droplet Digital PCR System, Bio-Rad's second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications. http://www.bio-rad.com/en-us/category/digital-pcr?WT.mc_id=sm-DBC-ww-qx200ddpcr_vyt_20160615_VnWaephMGm4"

Analytical Techniques

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