Precise Counting of Targeted Nucleic Acids Has Never Been Easier



February 22, 2017

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  • "For more info, visit Droplet Digital™ PCR is an elegant technology that permits accurate and absolute quantification of target nucleic acid molecules. By partitioning a PCR reaction into thousands of nanoliter-sized droplets followed by amplification and subsequent interrogation of each droplet, Droplet Digital PCR (ddPCR™) assays allow increased quantitative resolution, enhanced detection of difficult-to-amplify targets, better reproducibility, and simplified data analysis. This webinar provides an overview of ddPCR technology from technical and workflow-related perspectives and reviews key applications such as copy number variation (CNV) analysis, rare mutation detection (RMD), and standard-free (stand-alone) absolute quantification of nucleic acids. Droplet Digital PCR technology is the most precise and sensitive digital PCR solution for a wide variety of applications, featuring flexible assay chemistry optimized for both hydrolysis probe and dye-based assays. Nanoliter droplet partitioning reduces bias from amplification efficiency differences and PCR inhibitors and enables exquisitely accurate target quantification in a convenient assay design, without the use of standard curves. The QX200™ Droplet Digital PCR System, Bio-Rad's second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications. Presenter: Francisco Bizouarn, Global Digital Applications Specialist, Bio-Rad Digital Biology Center Francisco joined Bio-Rad in 2000 to support quantitative PCR (qPCR) technology as a Field Applications Specialist (FAS) in the southeast United States. In 2006, he moved into the role of International FAS for the Gene Expression Division to promote best practices in qPCR around the world. Francisco is one of Bio-Rad’s most experienced qPCR specialists and a renowned speaker at international conferences and congresses. Currently, he’s focused on quantitation and detection applications that take advantage of the high resolution and sensitivity provided by Droplet Digital PCR technology."

    Analytical Techniques

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